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Image that your agar plate is divided into 4 quadrants. Inoculate the specimen onto the first quadrant of the plate. | |
| Flame the inoculating loop until it is red hot. Allow it to cool. | ||
| Streak (lightly rub the loop over the specimen) for isolation on the first quadrant. | ||
| Turn the agar plate 1/4 of a turn in your hand. Go back into the first streak zone two to three times with your loop. Then streak for isolation in the second quadrant. | ||
Good isolation. |
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Turn the agar plate another 1/4 of a turn. Go back into the second streak zone two to three times with your loop. Then streak for isolation in the third quadrant. | |
Poor isolation. |
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Turn the agar plate one more time. Go back into the third
quadrant two to three times with your loop. Then streak for isolation
in the fourth quadrant. Do not touch your loop to the first quadrant. |
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No isolation. |
