| Inoculation of Broth |
If a broth medium is to be inoculated, it should
be inoculated before the agar plates. |
1.
broth
2. agar plate
3. smear
|
|
| While keeping the sample hand still, bring the broth towards it. Use your
little finger to remove the cap from the broth. The little
finger is finger number 5. |
|
| Sterilize the top of broth tube by passing it quickly
through a flame two to three times. |
|
| Carefully place the sample swab, loop, etc. into the
broth tube without touching the lip of the tube. Gently swirl the sample
swab, loop, etc. in the broth. Remove from broth. |
|
| Sterilize the top of the tube as before. |
|
| Replace the cap on the tube. |
|
