Genetic Engineering -

• Can we change our genes?
• Do we want to change our genes?

Tools of the gene engineer

• Splicing Enzymes
  • Restriction Enzymes - Flash model - Modification
  • Ligation Enzyme
• Cloning vectors
  • Plasmids
  • Cosmids
  • BACs and YACs
• DNA for Cloning
  • Genomic
  • cDNA
• Building libraries
• Screening libraries
• Cataloging your library
• Reading the elements in your library
• PCR - Polymerase Chain Reaction
  • Flash 4 rendition
  • details of PCR
  • Cycle sequencing
• Public Libraries

Text iGenetics by Peter J. Russell

This web site is provided for instruction in Botany and Zoology 342

by Kenneth G. Wilson,
Professor of Botany
Miami University
wilsonkg@muohio.edu