
How to find clones from a genomic library or gene bank?
EM Southern developed the proceedure for finding genes in digests
of DNA. It was modified and has become one of the most widely known techniques.
- Separate the digested or restricted DNA on an agarose gel using electrophoresis.
- Prepare a membrane replica of the gel by transferring
the separated DNA to nylon membrane, hybridize with known probe and develop
- Autoradogram. Similarly you can detect
for RNA (Northern Blot). You can detect proteins using antibodies (Western
Blot).
- Results of a Southern Blot
- Comparison of a Northern and Southern Blot
or hybridization
Text iGenetics by Peter J. Russell
This web site is provided for
instruction in Botany and Zoology 342
by Kenneth G. Wilson,
Professor of Botany
Miami University
wilsonkg@muohio.edu