Which one is the donor and which one is the recipient?

Alternatives to sex

what are the expect results of the following cross?

c+ b+ a+ x c- b- a-

Mapping by interrupted conjugation

Lets make the following Cross

Mix Cells and interrupted by agitating very vigorously and plate on Streptomycin
What is the role of Streptomycin in this experiment?

What should we conclude?

Conclusion

High-Resolution Mapping by Recombinant Frequency

Selection for leu+ in cross wild type x leu-, arg-, met-

F factors carrying bacterial genes

Transformation again

Bacteriaphage Genetics

T4 -

• EM Graph
• Lytic cycle
• Phage Cross
  • Results
  • Explanation
  • RF=(h+r+)+(h-r-)/total plaques

Lambda - lysogenic

• Integration

Transduction - The transformation of genetic material via a virus

• Generalized Transduction
• Specialized Transduction

Bacterial Alternatives to Sex

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This web site is provided for instruction in Botany and Zoology 342

by Kenneth G. Wilson,
Professor of Botany
Miami University
wilsonkg@muohio.edu