Sequencing DNA: Principle Create a nested sequence of fragments that can be seperated using gel electrophoresis to determine the sequence of nucleotides.

• Maxim and Gilbert degradation techniques using chemical mutagens.
• Seperate on gels
• Reconstructing the sequence from the nested fragments
• A real autoradiograph.
• Sanger creates a nested set of fragments using dideoxy nucleotide phosphates.
• Using DNA replicating enzymes and dideoxy nucleotides we can get a nice series of nested fragments.
• A real Sanger dideoxy autoradiograph.
• How do you get all lanes in one?
• Automated Sequencer Printout.
• Why are fluorescing dyes important to this process?
  • Dual labeling experiments - what does this mean to you.
• Computer scanning for ORFs in all six reading frames.
  • What are the six reading frames?
• Archibald Garrod - 1902 - Alkaptonuria

Text iGenetics by Peter J. Russell

This web site is provided for instruction in Botany and Zoology 342

by Kenneth G. Wilson,
Professor of Botany
Miami University
wilsonkg@muohio.edu