Therapeutic Cloning
lGenerates tissue, not an individual
lFirst step is to remove the haploid nucleus of an egg
cell and inject into the enucleated egg the
diploid nucleus of a somatic cell.
lThe somatic cell nucleus used would ideally be from a
cell of the patient. Why?
lThat fusion cell is then forced to divide and go through
cleavage until it reaches the blastocyst
embryonic stage. (Hollow ball with ICM)
lEmbryonic stem cell is taken from the growing embryo and
allowed to grow on an artificial feeder layer
that supports growth.
lFinally, appropriate molecules are added to the growing
cell line to allow differentiation
into the desired tissue.
lResulting tissue is genetically the same as the tissue
in patients body, no genetic
rejection.