Additional Markers Used in Mapping

Starch Gel Electrophoresis Coupled with Enzyme Assays

• Hemoglobin variants are co-cominant like sickle cell
• Esterase Isozyme markers - co-dominant like sickle cell

DNA characters are generally codominant

DNA Finger Print Bands as Markers

RAPD - (rapids) are not co-dominant

Polymerase Chain Reaction (PCR) - in vitro amplification of DNA

Randomly Amplified Pieces of DNA -RAPD (rapid)

RAPD gel

Mapping the DNA

Restriction Map

End Label Mapping

 Sites on the world wide web of related interest

http://www.perkin-elmer.com/ab/index.htm

This web site is provided for instruction in Botany and Zoology 342

by Kenneth G. Wilson,
Professor of Botany
Miami University
wilsonkg@muohio.edu