Genetic 342 A&B Kenneth G Wilson Professory of Botany

Study Sheet 3 for the third examination

Centra dogma

Transcription

Translation

Modification

cybernetic

Chemical changes

Code Cracking

# of letters

# of letters/word

# of words

four letter words in English

dublet code

triplet code

Har Gobind Khorana

Francis Crick

Homo polymers

Poly U

PNP

Marianne Grunberg-Manago

Severo Ochoa

reaction mixture for Homopolymers

Marshall Nirenberg

J. Heinrich Matthaei

Mixed homo-polymer

reaction mixture for Mixed
Homo-polymer (MHP) experiments

Expected results MCP

Repeating Co-polymers

reaction mixtures for Repeating Co Polymers

triplet binding assay TBA

reaction mixture

Players in TBA

table

start codon

first amino acid

stop codon

which base in code contains the most information?

how is the code read

Mutation

Silent mutation

Missense mutation

Nonsense mutation

Frame shift mutation

Base insertion

Base deletion

Triplet code proofs

Forward mutation

Reverse (or back) mutation

# of possible mutation of a single codon

Transition mutation

Transversion mutation

Suppresser mutation

Nonsense Suppresser Mutation

Bernstein mutation

amber mutation

opel mutation

ochre mutation

Sidney Brenner

Conditional lethal mutations

auxotroph

prototroph

High temperature sensitive mutation

Low temperature sensitive mutation

Mobile elements

Activator

Dissociater

Promoter

RNA polymerase

Holoenzyme

Core enzyme

Sigma factor

Rho

TTBACAT

TATAAT

tRNA

mRNA

rRNA

siRNA

miRNA

dicer

70s

80s

60s

50s

40s

5s

ribosomes (know in detail)

NOR

codon

anticodon

loaded tRNA

Reactants in Translation

Reactants in transcription

Capping

Tailing

Splicing

Intron

group I

group II

Splicosomal

Editing

Exon

Alternate splicing

Lactose

Galactose

Glucose

Beta- Galactosidase

Permease

Transacetylase

Repressor

IPTG

Allolactose

Operator

Operon

Cyclic AMP

Catobolite activator protein

cis acting

trans acting

induction

activation

repression

Restriction Enzymes

Modification

Vector

plasmid

BAC

YAC

pBR322

pUC18

genomic DNA

c DNA

Libraries

Sequencing DNA

Maxam and Gilbert

F Sanger

dideoxy

PCR

reactants for PCR

reactants for DNA sequencing dideoxy

Oliver genetics

This is our friend Oliver the parrotlet, Oliver is a true parrot, the smallest in the world.