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Genetic 342 A&B Kenneth G Wilson Professory of Botany |
Replication of DNA
Three possible models for semiconservative replication of DNA.
Test of models using Tritiated [3H] thymidine
How could you use the above model to measure the rate of DNA synthesis?
Now let us look at a model to explain the rest of the data.
The replication bubble in Drosophila embryos
The system is very similar in E. coli
Bidirectional replication of circular molecules give rise to theta structures and finally two circles
Origin |
Growing Regions |
Structure |
Results |
Two |
Two |
Ends |
No |
One |
One |
Forks |
Very Rare |
One |
Two |
Forks |
Common |
General Scheme for DNA replication
In vitro Enzymatic Replication of DNA
Chemistry of Chain elongation reaction catalyzed by DNA Polymerase.
Model for bidirectional migration of two forks
Origin of Replication - OriC
Origins of replication in E. coli
Autonomous Replicating Sequences in Yeast
What does it mean to prime the pump?
Primase starts the synthesis of DNA
Both strands are both Leading and Lagging
The structure of the growing forks
Ligase hooks things together
How can the polymerase go both directions at once ?
Lets look at a model to explain this part a, part b, and summary
This is our friend Oliver the parrotlet, Oliver is a true parrot, the smallest in the world. |