SUDAN IV
 
SPECIFIC FOR DIAGNOSTIC COLOR STAINING PROCEDURE STAINING TIME
PREPARATION
Suberin

Cutin

Reddish-orange

Red 

1.  Immerse sections in staining reagent in staining dish.

2.  Wash sections in 50% Ethanol in staining dish.

3.  Mount sections in glycerine on slide.

4.  Cover with coverslip.

1.  ca. 20 min.
 
 
 
 
 
 
 
 
 
1.  0.5 g Sudan IV in 100 ml of 80% Ethanol.

2.  50% Ethanol

3.  Glycerine
 

IMPORTANT NOTES: